Scholars have studied how entrepreneurs acquire resources but have not examined how resources may be bundled with constraints, which can threaten entrepreneurial autonomy. Organizational sponsors, such as incubators and accelerators, provide entrepreneurs with resources, but how do entrepreneurs sustain autonomy while seeking resources and support? We studied five entrepreneurial firms in a business incubator over a six-month period. While benefitting from incubator resources, entrepreneurs also experienced unexpected constraints, including mentor role conflict, gatekeeper control, and affiliation dissonance. By showing how entrepreneurs unbundled the incubator’s resources from constraints, we explain how entrepreneurs manage the tension between acquiring resources and preserving autonomy.