Online from: 1995
Subject Area: Mechanical & Materials Engineering
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|Title:||Characterisation of rapid prototyping techniques for studies in cell behaviour|
|Author(s):||P.S. Maher, (Division of Mechanical Engineering and Mechatronics, Dundee University, Dundee, UK), R.P. Keatch, (Division of Mechanical Engineering and Mechatronics, Dundee University, Dundee, UK), K. Donnelly, (Division of Mechanical Engineering and Mechatronics, Dundee University, Dundee, UK)|
|Citation:||P.S. Maher, R.P. Keatch, K. Donnelly, (2010) "Characterisation of rapid prototyping techniques for studies in cell behaviour", Rapid Prototyping Journal, Vol. 16 Iss: 2, pp.116 - 123|
|Keywords:||Cytology, Flow, Histology, Rapid prototypes, Thermoplasticity|
|Article type:||Research paper|
|DOI:||10.1108/13552541011025834 (Permanent URL)|
|Publisher:||Emerald Group Publishing Limited|
|Acknowledgements:||The authors thank Engineering and Physical Sciences Research Council (EPSRC) for funding this research. A special thanks to all the staff from Dundee University that have contributed to this research.|
Purpose – The area of microfluidic systems has greatly enhanced the
Design/methodology/approach – Using a Thermojet and Spectrum Z510 printer pre-designed micro-assays incorporating different size microchannels are dispensed. The base materials of both methods are examined for cytotoxic effects while in solution with primary tendon fibroblasts (PFB) cells. After obtaining favorable results from the toxicology experiments, PFB cells are seeded onto the thermojet structures with a view to investigate cell adherence, encapsulation and how the channel width influences cell alignment.
Findings – This research concluded that the thermojet had a higher degree of accuracy when manufacturing structures that incorporate microchannels when compared with the Spectrum Z510. Both techniques show that the accuracy of the build decreases with reduction in channel width. The fact that the Spectrum Z510 structures have to be infiltrated with a hardening glue as a post-processing technique (since the dispensed material is water-based and hence soluble) causes a cytotoxic effect compared to the thermojet plastic which is not cytotoxic in solution with PFB cells. Seeding the PBF cells directly onto the thermoplastic structure caused problems due to the hydrophobic nature of the material and this necessitated the technique of soaking the structures in a collagen bath to penetrate the surface and reduce the interactions of hydrophobic species enhancing cell attachment and proliferation. Without this coating the thermojet structures induced strong hydrophobic interactions at the surfaces of the microchannels with the culture media resulting in non-attachment and poor cell mortality.
Originality/value – This research paper describes a comparison between the base materials and methodology of two 3D printing techniques for applications in basic biological studies. This is achieved by analysing the dispensing accuracy of both technologies and the interaction between cells and surface at the interface.
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