Fluorescence lifetime technique on new detector

Citation

(2003), "Fluorescence lifetime technique on new detector", Pigment & Resin Technology, Vol. 32 No. 3. https://doi.org/10.1108/prt.2003.12932cab.002

Fluorescence lifetime technique on new detector

Fluorescence lifetime technique on new detector

Keywords: Microplates, Methods

Tecan (UK) has announced the further development of the highly successful Ultra microplate detection system by adding another detection technique – fluorescence lifetime.

The revolutionary Ultra Evolution instrument now offers all the high performance detection modes and reliability of the existing Ultra family, plus fluorescence lifetime measurement capabilities for samples in microplates, making it unique and accessible to all screening laboratories.

The Ultra Evolution supports a wide range of applications that may be performed in diverse settings including: target identification or lead discovery groups, assay development groups, high throughput screening facilities, secondary and confirmatory screening groups and contract research/screening companies.

By offering fluorescence lifetime as an additional detection mode, the new instrument provides scientists with a valuable tool when their assay results are hindered by compound auto-fluorescence.

Tecan has proved that the fluorescent signal from compounds can be distinguished from that of the label, even when it is present in excess and fluorescence at the same or similar wavelength as the label. Consequently, the Ultra Evolution provides screening laboratories with the chance to reduce the number of false positives they see during a primary screen.

This development addresses a critical bottleneck seen in assay development and HTS laboratories. Tecan is able to offer customers a choice of two laser excitation sources (red at 635 nm, blue at 440 nm) with the Fluorescence Lifetime module. Also available with Ultra Evolution is true bottom reading detection.

New optical systems have been added to the instrument, which now offers even more flexibility to users. With the increase in the number of cell-based assays and other assays that may not be read from the top of the well, the Ultra Evolution will become an even more powerful detection tool than before. With the new optical design, Tecan has retained the user-friendly "z" axis optimisation feature that scientists already use in top reading mode.

Furthermore, a unique multi-check quality control (QC) plate will also be offered with the system. It offers absorbance, fluorescence intensity and fluorescence polarisation detection and accuracy checks, as well as a further plate transport positioning check function. All round, the Ultra Evolution represents the best in microplate detection technology.

The unique fluorescence lifetime module may even be added as an upgrade at a later date, should the needs of the customer change.